Name: Laminin γ-2 Polyclonal Antibody
SKU: WP15424
Price: 268 USD
Availability: InStock

Product details

Background:

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological func

Specifications

Size	50μL/100μL
Target	Laminin γ-2
Reactivity	Human, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	135kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from the C-terminal region of human LAMC2. AA range:1021-1070
Specificity	Laminin γ-2 Polyclonal Antibody detects endogenous levels of Laminin γ-2 protein.
Gene Name	LAMC2
Protein Name	Laminin subunit gamma-2
Other Name	LAMC2; / LAMB2T; / LAMNB2; / Laminin subunit gamma-2; / Cell-scattering factor 140 kDa subunit; / CSF 140 kDa subunit; / Epiligrin subunit gamma; / Kalinin subunit gamma; / Kalinin/nicein/epiligrin 100 kDa subunitLadsin 140 kDa subunit; / Laminin B2t chain; / Laminin-5 subunit gamma; / Large adhesive scatter factor 140 kDa subunit; / Nicein subunit gamma
Species	Human
Gene ID	3918
Uniprot	Q13753,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q61092
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
Tissue Expression	The large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues. In 17-week old embryo the small variant is found in cerebral cortex, lung, and distal tubes of kidney, but not in epithelia except for distal tubuli.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> ECM-receptor interaction
Research Areas	>>PI3K-Akt signaling pathway; / >>Focal adhesion; / >>ECM-receptor interaction; / >>Toxoplasmosis; / >>Amoebiasis; / >>Human papillomavirus infection; / >>Pathways in cancer; / >>Small cell lung cancer
Function	Disease:Defects in LAMC2 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.,Domain:Domain IV is globular.,Domain:The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.,Function:Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.,miscellaneous:Binds heparin.,similarity:Contains 1 laminin IV type A domain.,similarity:Contains 8 laminin EGF-like domains.,subcellular location:Major component.,subunit:Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Gamma-2 is a subunit of laminin-5 (epiligrin/kalinin/nicein).,tissue specificity:The large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues. In 17-week old embryo the small variant is found in cerebral cortex, lung, and distal tubes of kidney, but not in epithelia except for distal tubuli.,