Price range: $268.00 through $328.00
Background:
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],
| Size | 50μL/100μL |
| Target | LARG |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 173kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARHGEF12. AA range:449-498 |
| Specificity | LARG Polyclonal Antibody detects endogenous levels of LARG protein. |
| Gene Name | ARHGEF12 |
| Protein Name | Rho guanine nucleotide exchange factor 12 |
| Other Name | ARHGEF12; / KIAA0382; / LARG; / Rho guanine nucleotide exchange factor 12; / Leukemia-associated RhoGEF |
| Species | Human |
| Gene ID | 23365 |
| Uniprot | Q9NZN5, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8R4H2 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Membrane . Translocated to the membrane upon stimulation. . |
| Tissue Expression | Ubiquitously expressed. Isoform 2 is found in jejunum and testis. |
| Signaling_pathway | Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Human Diseases >> Cancer: overview >> Pathways in cancer |
| Research Areas | >>Vascular smooth muscle contraction; / >>Axon guidance; / >>Platelet activation; / >>C-type lectin receptor signaling pathway; / >>Regulation of actin cytoskeleton; / >>Pathogenic Escherichia coli infection; / >>Yersinia infection; / >>Tuberculosis; / >>Human cytomegalovirus infection; / >>Pathways in cancer; / >>Proteoglycans in cancer |
| Function | Disease:A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11; 11)(q23; 23) with MLL.,Function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane upon stimulation.,subunit:Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain with IGF1R beta subunit.,tissue specificity:Ubiquitously expressed. Isoform 2 is found in jejunum and testis., |
