U-Blot® LC3A Rabbit mAb
WR4052
WR4052 Immunohistochemical analysis of paraffin-embedded rat colon tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4052 Immunohistochemical analysis of paraffin-embedded mouse prostate tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4052 Western blot analysis of lysates from Mouse brain cells, using LC3A Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012],
Specifications
| Size | 50μL/100μL |
| Target | LC3A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 14kD |
| MW(Observed) | 16kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0130R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MAP1LC3A |
| Protein Name | LC3A |
| Other Name | Microtubule-associated proteins 1A/1B light chain 3A; / Autophagy-related protein LC3 A; / Autophagy-related ubiquitin-like modifier LC3 A; / MAP1 light chain 3-like protein 1; / MAP1A/MAP1B light chain 3 A; / MAP1A/MAP1B LC3 A; / Microtubule-associated protein 1 light chain 3 alpha |
| Species | Human |
| Gene ID | 84557 |
| Uniprot | Q9H492, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q91VR7, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q6XVN8 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cell growth and death >> Ferroptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Mitophagy - animal; / >>Autophagy - animal; / >>Ferroptosis; / >>Apelin signaling pathway; / >>NOD-like receptor signaling pathway; / >>Amyotrophic lateral sclerosis; / >>Pathways of neurodegeneration - multiple diseases; / >>Shigellosis; / >>Kaposi sarcoma-associated herpesvirus infection |
| Function | Function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes., |
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