Price range: $268.00 through $328.00
Background:
LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | LEO1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | 73kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human LEO1 AA range: 159-209 |
| Specificity | This antibody detects endogenous levels of LEO1 at Human/Mouse/Rat |
| Gene Name | LEO1 RDL |
| Protein Name | LEO1 |
| Other Name | |
| Species | Human |
| Gene ID | 123169 |
| Uniprot | Q8WVC0, |
| Species.1 | Mouse |
| Gene ID.1 | 235497 |
| Uniprot.1 | Q5XJE5, |
| Species.2 | Rat |
| Gene ID.2 | 300837 |
| Uniprot.2 | Q641X2 |
| Cellular Localization | Nucleus . |
| Tissue Expression | Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. May be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.,similarity:Belongs to the LEO1 family.,subunit:Component of the PAF1 complex, which consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73.,tissue specificity:Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver., |
