LIMK-1/2 [PTR2545] mouse mAb
Ucallm_WM1335-50
Price range: $268.00 through $328.00
Product details
Background:
LIM domain kinase 1(LIMK1) Homo sapiens There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cog
Specifications
| Size | 50μL/100μL |
| Target | LIMK-1/2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 68kD,72kD |
| Host Species | Mouse |
| Isotype | IgG1,Lambda |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMMR2545 |
| Immunogen | Synthesized peptide derived from human LIMK-1/2 AA range: 500-600 |
| Specificity | This antibody detects endogenous levels of LIMK-1/2 protein. |
| Gene Name | LIMK1 LIMK |
| Protein Name | LIM domain kinase 1 (LIMK-1) (EC 2.7.11.1) |
| Other Name | LIM domain kinase 1; / LIMK-1 |
| Species | Human |
| Gene ID | 3984;3985 |
| Uniprot | P53667;P53671, |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P53669 |
| Cellular Localization | |
| Tissue Expression | Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. |
| Signaling_pathway | Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis |
| Research Areas | >>Axon guidance; / >>Fc gamma R-mediated phagocytosis; / >>Regulation of actin cytoskeleton; / >>Yersinia infection; / >>Human immunodeficiency virus 1 infection |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Disease:Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,Function:Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.,PTM:Autophosphorylated.,PTM:Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 LIM zinc-binding domains.,subunit:Self-associates. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with SSH1. Interacts with NISCH.,tissue specificity:Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle., |
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