Name: U-Blot® LRP1 Rabbit mAb
SKU: WR4124
Price: 268 USD
Availability: InStock

U-Blot® LRP1 Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4124 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4124 Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded rat colon tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4124 Immunohistochemical analysis of paraffin-embedded rat colon tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4124 Rat brain

Western blot analysis of lysates from A549 cells, using LRP1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4124 Western blot analysis of lysates from A549 cells, using LRP1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

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50μl

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SKU: WR4124-50

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Product details

Background:This gene encodes a member of the low-density lipoprotein receptor family of proteins. The encoded preproprotein is proteolytically processed by furin to generate 515 kDa and 85 kDa subunits that form the mature receptor (PMID: 8546712). This receptor is involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. In addition, the encoded protein is necessary for the alpha 2-macroglobulin-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of this gene decreases with age and has been found to be lower than controls in brain tissue from Alzheimer's disease patients. [provided by RefSeq, Oct 2015],

Specifications

Size	50μL/100μL
Target	LRP1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	85kD
MW(Observed)	85kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0231R
Immunogen	C-ternal
Specificity	Endogenous
Gene Name	LRP1
Protein Name	Prolow-density lipoprotein receptor-related protein 1
Other Name	LRP1; / A2MR; / APR; / Prolow-density lipoprotein receptor-related protein 1; / LRP-1; / Alpha-2-macroglobulin receptor; / A2MR; / Apolipoprotein E receptor; / APOER; / CD antigen CD91
Species	Human
Gene ID	4035
Uniprot	Q07954,
Species.1	Mouse
Gene ID.1	16971
Uniprot.1	Q91ZX7
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Membranous
Tissue Expression	Most abundant in liver, brain and lung.
Signaling_pathway	Human Diseases >> Neurodegenerative disease >> Alzheimer disease
Research Areas	>>Cholesterol metabolism; / >>Alzheimer disease; / >>Malaria
Function	Function:Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.,PTM:Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.,PTM:Phosphorylated on serine and threonine residues.,PTM:Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.,PTM:The N-terminus is blocked.,similarity:Belongs to the LDLR family.,similarity:Contains 22 EGF-like domains.,similarity:Contains 31 LDL-receptor class A domains.,similarity:Contains 34 LDL-receptor class B repeats.,subcellular location:After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus.,subunit:Heterodimer of an 85-kDa membrane-bound carboxyl subunit and a non-covalently attached 515-kDa amino-terminal subunit. Intracellular domain interacts with MAFB (By similarity). Found in a complex with PID1/PCLI1, LRP1 and CUBNI. Interacts with SNX17, PID1/PCLI1, PDGF and CUBN. The intracellular domain interacts with SHC1, GULP1 and DAB1. Interacts with LRPAP1.,tissue specificity:Most abundant in liver, brain and lung.,