Price range: $268.00 through $328.00
Background:
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | MAFA |
| Reactivity | Human, Mouse |
| Applications | WB |
| MW(Calculated) | |
| MW(Observed) | 38kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human MAFA AA range: 204-254 |
| Specificity | This antibody detects endogenous levels of MAFA at Human/Mouse |
| Gene Name | MAFA |
| Protein Name | MAFA |
| Other Name | Transcription factor MafA; / Pancreatic beta-cell-specific transcriptional activator; / Transcription factor RIPE3b1; / V-maf musculoaponeurotic fibrosarcoma oncogene homolog A |
| Species | Human |
| Gene ID | 389692 |
| Uniprot | Q8NHW3, |
| Species.1 | Mouse |
| Gene ID.1 | 378435 |
| Uniprot.1 | Q8CF90 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Expressed in the islets of Langerhans (at protein level). |
| Signaling_pathway | |
| Research Areas | >>Type II diabetes mellitus; / >>Maturity onset diabetes of the young |
| Function | Function:Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.,induction:Up-regulated by glucose.,PTM:Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.,PTM:Ubiquitinated, leading to its degradation by the proteasome.,similarity:Belongs to the bZIP family. Maf subfamily.,similarity:Contains 1 bZIP domain.,subcellular location:Detected in nuclei of pancreas islet beta cells.,subunit:Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1., |
