Price range: $268.00 through $328.00
Background:
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Malic enzyme 1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 64kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ME1. AA range:468-517 |
| Specificity | Malic enzyme 1 Polyclonal Antibody detects endogenous levels of Malic enzyme 1 protein. |
| Gene Name | ME1 |
| Protein Name | NADP-dependent malic enzyme |
| Other Name | ME1; / NADP-dependent malic enzyme; / NADP-ME; / Malic enzyme 1 |
| Species | Human |
| Gene ID | 4199 |
| Uniprot | P48163, |
| Species.1 | Mouse |
| Gene ID.1 | 17436 |
| Uniprot.1 | P06801, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P13697 |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Expressed in all tissues tested including liver, placenta and white adipose tissue. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> PPAR signaling pathway |
| Research Areas | >>Pyruvate metabolism; / >>Metabolic pathways; / >>Carbon metabolism; / >>PPAR signaling pathway |
| Function | Catalytic activity:(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.,cofactor:Divalent metal cations. Prefers magnesium or manganese.,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the malic enzymes family.,subunit:Homotetramer.,tissue specificity:Expressed in all tissues tested including liver, placenta and white adipose tissue., |
