Name: MaxiKβ Polyclonal Antibody
SKU: WP13093
Price: 268 USD
Availability: InStock

Product details

Background:

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which slows activation kinetics, leads to steeper calcium sensitivity, and shifts the voltage range of current activation to more negative potentials than does the beta 1 subunit. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	MaxiKβ
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	24kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human MaxiKbeta. AA range:70-119
Specificity	MaxiKβ Polyclonal Antibody detects endogenous levels of MaxiKβ protein.
Gene Name	KCNMB4
Protein Name	Calcium-activated potassium channel subunit beta-4
Other Name	KCNMB4; / Calcium-activated potassium channel subunit beta-4; / BK channel subunit beta-4; / BKbeta4; / Hbeta4; / Calcium-activated potassium channel; / subfamily M subunit beta-4; / Charybdotoxin receptor subunit beta-4; / K; / VCA; / beta-4; / Maxi K channel sub
Species	Human
Gene ID	27345
Uniprot	Q86W47,
Species.1	Mouse
Gene ID.1	58802
Uniprot.1	Q9JIN6,
Species.2	Rat
Gene ID.2	66016
Uniprot.2	Q9ESK8
Cellular Localization	Membrane; Multi-pass membrane protein.
Tissue Expression	Predominantly expressed in brain. In brain, it is expressed in the cerebellum, cerebral cortex, medulla, spinal cord, occipital pole, frontal lobe, temporal lobe, putamen, amygdala, caudate nucleus, corpus callosum, hippocampus, substantia nigra and thalamus. Weakly or not expressed in other tissues.
Signaling_pathway	Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway
Research Areas	>>cGMP-PKG signaling pathway; / >>Vascular smooth muscle contraction; / >>Insulin secretion
Function	Domain:Resistance to charybdotoxin (CTX) toxin is mediated by the extracellular domain.,Function:Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. May decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. Makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations.,miscellaneous:Treatment with okadaic acid reduces its effect on KCNMA1.,PTM:N-glycosylated. A highly glycosylated form is promoted by KCNMA1. Glycosylation, which is not required for the interaction with KCNMA1 and subcellular location, increases protection against charybdotoxin.,PTM:Phosphorylated. Phosphorylation modulates its effect on KCNMA1 activation kinetics.,similarity:Belongs to the KCNMB family.,subunit:Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB4 per KCNMA1 tetramer.,tissue specificity:Predominantly expressed in brain. In brain, it is expressed in the cerebellum, cerebral cortex, medulla, spinal cord, occipital pole, frontal lobe, temporal lobe, putamen, amygdala, caudate nucleus, corpus callosum, hippocampus, substantia nigra and thalamus. Weakly or not expressed in other tissues.,