Price range: $268.00 through $328.00
Background:This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],
| Size | 50μL/100μL |
| Target | Mcl-1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 37kD |
| MW(Observed) | 37kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0585R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MCL1 |
| Protein Name | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| Other Name | MCL1; / BCL2L3; / Induced myeloid leukemia cell differentiation protein Mcl-1; / Bcl-2-like protein 3; / Bcl2-L-3; / Bcl-2-related protein EAT/mcl1; / mcl1/EAT |
| Species | Human |
| Gene ID | 4170 |
| Uniprot | Q07820, |
| Species.1 | Mouse |
| Gene ID.1 | 17210 |
| Uniprot.1 | P97287, |
| Species.2 | Rat |
| Gene ID.2 | 60430 |
| Uniprot.2 | Q9Z1P3 |
| Cellular Localization | Cytoplasm, Nucleus |
| Tissue Expression | Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>PI3K-Akt signaling pathway; / >>Apoptosis; / >>JAK-STAT signaling pathway; / >>MicroRNAs in cancer |
| Function | Function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.,similarity:Belongs to the Bcl-2 family.,subcellular location:Cytoplasmic, associated with mitochondria.,subunit:Interacts with BAD, BOK, BIK and BFM (By similarity). Interacts with PMAIP1. Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptototic BCL2-related proteins., |
