MCM3 (Phospho Ser112) rabbit pAb
Ucallm_WP10413-50
Price range: $288.00 through $368.00
Product details
Background:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012],
Specifications
| Size | 50μL/100μL |
| Target | MCM3 Phospho Ser112 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | |
| MW(Observed) | 89kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized phosho peptide around human MCM3 (Ser112) |
| Specificity | This antibody detects endogenous levels of Human MCM3 (phospho-Ser112) |
| Gene Name | MCM3 |
| Protein Name | MCM3 (Ser112) |
| Other Name | MCM3; / DNA replication licensing factor MCM3; / DNA polymerase alpha holoenzyme-associated protein P1; / P1-MCM3; / RLF subunit beta; / p102 |
| Species | Human |
| Gene ID | 4172 |
| Uniprot | P25205, |
| Species.1 | Mouse |
| Gene ID.1 | 17215 |
| Uniprot.1 | P25206 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. . |
| Tissue Expression | Cervix,Coronary artery,Epithelium,Lymph,Osteosarcoma,Pancre |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle |
| Research Areas | >>DNA replication; / >>Cell cycle |
| Function | Function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.,miscellaneous:Associated with the replication-specific DNA polymerase alpha.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain., |
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