Name: MEK-4 Polyclonal Antibody
SKU: WP13135
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Specifications

Size	50μL/100μL
Target	MEK-4
Reactivity	Human, Mouse, Rat
Applications	WB, ELISA, IHC
MW(Calculated)
MW(Observed)	44kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human SEK1/MKK4. AA range:46-95
Specificity	MEK-4 Polyclonal Antibody detects endogenous levels of MEK-4 protein.
Gene Name	MAP2K4 JNKK1 MEK4 MKK4 PRKMK4 SEK1 SERK1 SKK1
Protein Name	Dual specificity mitogen-activated protein kinase kinase 4
Other Name	Dual specificity mitogen-activated protein kinase kinase 4; / MAP kinase kinase 4; / MAPKK 4; / JNK-activating kinase 1; / MAPK/ERK kinase 4; / MEK 4; / SAPK/ERK kinase 1; / SEK1; / Stress-activated protein kinase kinase 1; / SAPK kinase 1; / SAPKK-1; / SAPKK1; / c-Jun N-terminal kinase kinase 1; / JNKK
Species	Human
Gene ID	6416
Uniprot	P45985,
Species.1	Mouse
Gene ID.1	26398
Uniprot.1	P47809
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm . Nucleus .
Tissue Expression	Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Signaling_pathway	Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Toll-like receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>TNF signaling pathway; / >>GnRH signaling pathway; / >>Relaxin signaling pathway; / >>Growth hormone synthesis, secretion and action; / >>Alcoholic liver disease; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Salmonella infection; / >>Yersinia infection; / >>Chagas disease; / >>Hepatitis B; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Epstein-Barr virus infection; / >>Chemical carcinogenesis - reactive oxygen species; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).,PTM:Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with SPAG9.,tissue specificity:Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.,