Name: MEK-6 Monoclonal Antibody
SKU: WM0317
Price: 268 USD
Availability: InStock

Product details

Background:
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	MEK-6
Reactivity	Human, Mouse
Applications	WB, IHC, IF, ELISA
MW(Calculated)	37kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	19H9
Immunogen	Purified recombinant fragment of human MEK-6 expressed in E. Coli.
Specificity	MEK-6 Monoclonal Antibody detects endogenous levels of MEK-6 protein.
Gene Name	MAP2K6
Protein Name	Dual specificity mitogen-activated protein kinase kinase 6
Other Name	MAP2K6; / MEK6; / MKK6; / PRKMK6; / SKK3; / Dual specificity mitogen-activated protein kinase kinase 6; / MAP kinase kinase 6; / MAPKK 6; / MAPK/ERK kinase 6; / MEK 6; / Stress-activated protein kinase kinase 3; / SAPK kinase 3; / SAPKK-3; / SAPKK3
Species	Human
Gene ID	5608
Uniprot	P52564,
Species.1	Mouse
Gene ID.1	26399
Uniprot.1	P70236
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus . Cytoplasm . Cytoplasm, cytoskeleton . Binds to microtubules.
Tissue Expression	Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>MAPK signaling pathway; / >>Rap1 signaling pathway; / >>Cellular senescence; / >>Osteoclast differentiation; / >>Toll-like receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>TNF signaling pathway; / >>Inflammatory mediator regulation of TRP channels; / >>GnRH signaling pathway; / >>Growth hormone synthesis, secretion and action; / >>Alcoholic liver disease; / >>Amyotrophic lateral sclerosis; / >>Pathways of neurodegeneration - multiple diseases; / >>Salmonella infection; / >>Yersinia infection; / >>Toxoplasmosis; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Probably activated by dual phosphorylation on Ser-207 and Thr-211.,Function:Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.,induction:Strongly activated by UV, anisomycin, and osmotic shock but not by phorbol esters, NGF or EGF.,PTM:Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.,PTM:Weakly autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with Yersinia yopJ.,tissue specificity:Isoform 2 is only expressed in skeletal muscle. Isoform 1, on the other hand, is found in skeletal muscle, heart, and in lesser extent in liver or pancreas.,