Name: MEK1 mouse mAb
SKU: WM0597
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	MEK-1
Reactivity	Human
Applications	WB
MW(Calculated)
MW(Observed)	45kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	6G5
Immunogen	Recombinant human MEK1 protein.
Specificity	This antibody detects endogenous levels of MEK1/2 and does not cross-react with related proteins.
Gene Name	MAP2K1
Protein Name
Other Name	AA589381; / Dual specificity mitogen-activated protein kinase kinase 1; / Dual specificity mitogen-activated protein kinase kinase 2; / ERK activator kinase 1; / ERK activator kinase 2; / FLJ26075; / MAP kinase kinase 1; / MAP kinase kinase 2; / MAP2K1; / MAP2K2; / MAPK/ERK kinase 1; / MAPK/ERK kinase 2; / MAPKK 1; / MAPKK1; / MAPKK2; / MEK 1; / MEKK1; / Mitogen activated protein kinase kinase 1; / Mitogen activated protein kinase kinase 2; / Mitogen-activated protein kinase kinase 2, p45; / MK2; / MKK 1; / MKK 2; / MKK1; / MKK2; / MP2K1_HUMAN; / PRKMK 1; / PRKMK 2; / Prkmk1; / Prkmk2; / protein kinase, mitogen-activated, kinase 1; / MAP kinase kinase 1; / Protein kinase, mitogen-activated, kinase 1; / Protein kinase, mitogen-activated, kinase 2.
Species	Human
Gene ID	5604
Uniprot	Q02750
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	extracellular region,nucleus,cytoplasm,mitochondrion,early endosome,late endosome,peroxisomal membrane,endoplasmic reticulum,Golgi apparatus,cytosol,microtubule,cell-cell junction,focal adhesio
Tissue Expression	Colon carcinoma,Epithelium,Human cerebellum,Muscle,Platelet
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Bladder cancer;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>HIF-1 signaling pathway; / >>FoxO signaling pathway; / >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>Apoptosis; / >>Cellular senescence; / >>Vascular smooth muscle contraction; / >>VEGF signaling pathway; / >>Apelin signaling pathway; / >>Gap junction; / >>Signaling pathways regulating pluripotency of stem cells; / >>Neutrophil extracellular trap formation; / >>Toll-like receptor signaling pathway; / >>Natural killer cell mediated cytotoxicity; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Long-term potentiation; / >>Neurotrophin signaling pathway; / >>Long-term depression; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>GnRH signaling pathway; / >>Estrogen signaling pathway; / >>Melanogenesis; / >>Prolactin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Oxytocin signaling pathway; / >>Relaxin signaling pathway; / >>GnRH secretion; / >>Cushing syndrome; / >>Growth hormone synthesis, secretion and action; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Salmonella infection; / >>Yersinia infection; / >>Hepatitis C; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Influenza A; / >>Human papillomavirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Endometrial cancer; / >>Glioma; / >>Prostate cancer; / >>Thyroid cancer; / >>Melanoma; / >>Bladder cancer; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Non-small cell lung cancer; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Central carbon metabolism in cancer; / >>Choline metabolism in cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Disease:Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.,Function:Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.,PTM:MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MORG1.,