Specifications
| Size | 50μL/100μL |
| Target | MEK5 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 49kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:500-2000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Monoclonal |
| Clone Number | 2G10 |
| Immunogen | Synthesized peptide derived from human MEK5 |
| Specificity | This antibody detects endogenous levels of MEK5 at Human, Mouse,Rat |
| Gene Name | MAP2K5 MEK5 MKK5 PRKMK5 |
| Protein Name | Dual specificity mitogen-activated protein kinase kinase 5 |
| Other Name | MAP2K5; / MEK5; / MKK5; / PRKMK5; / Dual specificity mitogen-activated protein kinase kinase 5; / MAP kinase kinase 5; / MAPKK 5; / MAPK/ERK kinase 5; / MEK 5 |
| Species | Human |
| Gene ID | 5607 |
| Uniprot | Q13163, |
| Species.1 | Mouse |
| Gene ID.1 | 23938 |
| Uniprot.1 | Q9WVS7, |
| Species.2 | Rat |
| Gene ID.2 | 29568 |
| Uniprot.2 | Q62862 |
| Cellular Localization | |
| Tissue Expression | Expressed in many adult tissues. Abundant in heart and skeletal muscle. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway |
| Research Areas | |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Domain:Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.,Function:Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.,PTM:Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.,PTM:Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 OPR domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI (By similarity). Interacts with Yersinia yopJ.,tissue specificity:Expressed in many adult tissues. Abundant in heart and skeletal muscle., |
