U-Blot® Methionyl Aminopeptidase 1 Rabbit mAb
WR4770 Western Blot analysis using A375 lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Price range: $268.00 through $328.00
Product details
Background:catalytic activity:Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.,cofactor:Binds 1 sodium ion per subunit. The sodium ion has a structural role.,cofactor:Binds 2 cobalt ions per subunit.,cofactor:Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions.,function:Removes the amino-terminal methionine from nascent proteins.,function:Removes the amino-terminal methionine from nascent proteins. Required for normal progression through the cell cycle.,similarity:Belongs to the peptidase M24A family.,
Specifications
| Size | 50μL/100μL |
| Target | Methionyl Aminopeptidase 1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 42kD |
| MW(Observed) | 42kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1130R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | METAP1 KIAA0094 |
| Protein Name | Methionine aminopeptidase 1 (MAP 1) (MetAP 1) (Peptidase M 1) |
| Other Name | AMPM1 HUMAN,MAP 1,MAP1A,MetAP 1,MetAP1A,Methionine aminopeptidase 1,Peptidase M 1, |
| Species | Human |
| Gene ID | 23173 |
| Uniprot | P53582, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8BP48 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Bone marrow,Placenta,Testis, |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.,cofactor:Binds 1 sodium ion per subunit. The sodium ion has a structural role.,cofactor:Binds 2 cobalt ions per subunit.,cofactor:Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions.,Function:Removes the amino-terminal methionine from nascent proteins.,Function:Removes the amino-terminal methionine from nascent proteins. Required for normal progression through the cell cycle.,similarity:Belongs to the peptidase M24A family., |
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