Mi2-α Monoclonal Antibody
Ucallm_WM0508-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Mi2-α |
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Pig |
| Applications | WB, IHC, IF, FC |
| MW(Calculated) | 227kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:2000; IHC 1:500-1:1000; IF 1:100-1:500; Flow Cyt 1:100-1:200; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 4A5 |
| Immunogen | Purified recombinant human Mi2-α (C-terminus) protein fragments expressed in E.coli. |
| Specificity | Mi2-α Monoclonal Antibody detects endogenous levels of Mi2-α protein. |
| Gene Name | CHD3 |
| Protein Name | Chromodomain-helicase-DNA-binding protein 3 |
| Other Name | CHD3; / Chromodomain-helicase-DNA-binding protein 3; / CHD-3; / ATP-dependent helicase CHD3; / Mi-2 autoantigen 240 kDa protein; / Mi2-alpha; / Zinc finger helicase; / hZFH |
| Species | Human |
| Gene ID | 1107 |
| Uniprot | Q12873 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus, PML body . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase and mitosis. . |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Genetic Information Processing >> Chromosome >> ATP-dependent chromatin remodeling |
| Research Areas | |
| Function | Disease:One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.,Function:Probable transcription regulator.,sequence Caution:Differs from position 1967 onward for unknown reasons.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts via its C-terminal region with HABP4.,tissue specificity:Widely expressed., |
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