Price range: $268.00 through $328.00
Background:This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | MIF |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 13kD |
| MW(Observed) | 13kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:3000-1:10000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0418R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MIF |
| Protein Name | Macrophage migration inhibitory factor |
| Other Name | MIF; / GLIF; / MMIF; / Macrophage migration inhibitory factor; / MIF; / Glycosylation-inhibiting factor; / GIF; / L-dopachrome isomerase; / L-dopachrome tautomerase; / Phenylpyruvate tautomerase |
| Species | Human |
| Gene ID | 4282 |
| Uniprot | P14174, |
| Species.1 | Mouse |
| Gene ID.1 | 17319 |
| Uniprot.1 | P34884, |
| Species.2 | Rat |
| Gene ID.2 | 81683 |
| Uniprot.2 | P30904 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Brain,Lens,Liver,Lung,Platelet,Skin |
| Signaling_pathway | |
| Research Areas | >>Tyrosine metabolism; / >>Phenylalanine metabolism; / >>Metabolic pathways |
| Function | Catalytic activity:Keto-phenylpyruvate = enol-phenylpyruvate.,Disease:Genetic variations in MIF are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement.,Function:The expression of MIF at sites of inflammation suggest a role for the mediator in regulating the function of macrophage in host defense. Also acts as a phenylpyruvate tautomerase.,similarity:Belongs to the MIF family.,subunit:Homotrimer. Interacts with COPS5 and BNIPL., |
