Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010],
| Size | 50μL/100μL |
| Target | MLL2 |
| Reactivity | Human, Mouse |
| Applications | IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 609kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-300; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 1430-1510 |
| Specificity | MLL2 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | MLL2 ALR KMT2D MLL4 |
| Protein Name | Histone-lysine N-methyltransferase MLL2 (ALL1-related protein) (Lysine N-methyltransferase 2D) (KMT2D) (Myeloid/lymphoid or mixed-lineage leukemia protein 2) |
| Other Name | |
| Species | Human |
| Gene ID | 8085 |
| Uniprot | O14686, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q6PDK2 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Expressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver. |
| Signaling_pathway | |
| Research Areas | >>Lysine degradation; / >>Metabolic pathways; / >>Cushing syndrome |
| Function | Catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.,Domain:LXXLL motifs 5 and 5 are essential for the association with ESR1 nuclear receptor.,Function:Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription.,miscellaneous:This gene mapped to a chromosomal region involved in duplications and translocations associated with cancer.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily.,similarity:Contains 1 post-SET domain.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 SET domain.,similarity:Contains 5 PHD-type zinc fingers.,subunit:Component of the MLL2/MLL3 complex (also named ASCOM complex), at least composed of MLL2, MLL3, ASH2L, RBBP5, DPY30, NCOA6, WDR5, MEN1, KDM6A and PAXIP1/PTIP. Interacts with NFE2. Interacts with ESR1; interaction is direct.,tissue specificity:Expressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver., |
