Name: MMP-9 Monoclonal Antibody
SKU: WM0323
Price: 268 USD
Availability: InStock

Product details

Background:
matrix metallopeptidase 9(MMP9) Homo sapiens Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	MMP-9
Reactivity	Human
Applications	WB, IHC, IF, FC, ELISA
MW(Calculated)	78kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	3F5
Immunogen	Purified recombinant fragment of human MMP-9 expressed in E. Coli.
Specificity	MMP-9 Monoclonal Antibody detects endogenous levels of MMP-9 protein.
Gene Name	MMP9
Protein Name	Matrix metalloproteinase-9
Other Name	MMP9; / CLG4B; / Matrix metalloproteinase-9; / MMP-9; / 92 kDa gelatinase; / 92 kDa type IV collagenase; / Gelatinase B; / GELB
Species	Human
Gene ID	4318
Uniprot	P14780,
Species.1	Mouse
Gene ID.1
Uniprot.1	P41245
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted, extracellular space, extracellular matrix .
Tissue Expression	Detected in neutrophils (at protein level) (PubMed:7683678). Produced by normal alveolar macrophages and granulocytes.
Signaling_pathway	Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Bladder cancer;Human Diseases >> Cancer: specific types >> Prostate cancer;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>Endocrine resistance; / >>IL-17 signaling pathway; / >>TNF signaling pathway; / >>Leukocyte transendothelial migration; / >>Estrogen signaling pathway; / >>Relaxin signaling pathway; / >>Hepatitis B; / >>Pathways in cancer; / >>Transcriptional misregulation in cancer; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Prostate cancer; / >>Bladder cancer; / >>Diabetic cardiomyopathy; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:Cleavage of gelatin types I and V and collagen types IV and V.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 3 calcium ions per subunit.,Disease:Defects in MMP9 may be a cause of susceptibility to lumbar disk herniation (LDH) [MIM:603932]. LDH is the predominant cause of low-back pain and unilateral leg pain.,Domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:Inhibited by histatin-3 1/24 (histatin-5).,Function:May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-\|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.,induction:Activated by 4-aminophenylmercuric acetate and phorbol ester.,miscellaneous:In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.,PTM:N- and O-glycosylated.,PTM:Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 3 fibronectin type-II domains.,similarity:Contains 4 hemopexin-like domains.,subunit:Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.,tissue specificity:Produced by normal alveolar macrophages and granulocytes.,