Name: U-Blot® MMP13 Rabbit mAb
SKU: WR4076
Price: 268 USD
Availability: InStock

U-Blot® MMP13 Rabbit mAb

WR4076

Western blot analysis of lysates from Hela cells, using MMP13 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4076 Western blot analysis of lysates from Hela cells, using MMP13 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4076-50

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Product details

Background:This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016],

Specifications

Size	50μL/100μL
Target	MMP-13
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)	54kD
MW(Observed)	60kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:100-500; WB 1:500-2000; IF 1:200-1000; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0161R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	MMP13
Protein Name	Collagenase 3
Other Name	MMP13; / Collagenase 3; / Matrix metalloproteinase-13; / MMP-13
Species	Human
Gene ID	4322
Uniprot	P45452,
Species.1	Mouse
Gene ID.1	17386
Uniprot.1	P33435
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted
Tissue Expression	Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue.
Signaling_pathway	Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway
Research Areas	>>IL-17 signaling pathway; / >>Relaxin signaling pathway; / >>Parathyroid hormone synthesis, secretion and action
Function	cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 4 calcium ions per subunit.,Disease:Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common.,Domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,Function:Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,tissue specificity:Seems to be specific to breast carcinomas.,