Mnk2 Polyclonal Antibody
Ucallm_WP13210-50
Price range: $268.00 through $328.00
Product details
Background:
MAP kinase interacting serine/threonine kinase 2(MKNK2) Homo sapiens This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex.
Specifications
| Size | 50μL/100μL |
| Target | MNK2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | |
| MW(Observed) | 51kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from the Internal region of human Mnk2. |
| Specificity | Mnk2 Polyclonal Antibody detects endogenous levels of Mnk2 protein. |
| Gene Name | MKNK2 GPRK7 MNK2 |
| Protein Name | MAP kinase-interacting serine/threonine-protein kinase 2 |
| Other Name | MKNK2; / GPRK7; / MNK2; / MAP kinase-interacting serine/threonine-protein kinase 2; / MAP kinase signal-integrating kinase 2; / MAPK signal-integrating kinase 2; / Mnk2 |
| Species | Human |
| Gene ID | 2872 |
| Uniprot | Q9HBH9, |
| Species.1 | Mouse |
| Gene ID.1 | 17347 |
| Uniprot.1 | Q8CDB0, |
| Species.2 | Rat |
| Gene ID.2 | 299618 |
| Uniprot.2 | Q5U2N4 |
| Cellular Localization | [Isoform 2]: Nucleus, PML body.; [Isoform 1]: Cytoplasm. |
| Tissue Expression | Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>HIF-1 signaling pathway; / >>Insulin signaling pathway |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 1 zinc ion per subunit.,cofactor:Magnesium.,Function:May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.,PTM:Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2. Isoform 2 interacts with ESR2.,tissue specificity:Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1., |
Please login to post questions
Related products
