Price range: $268.00 through $328.00
Background:
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Moesin/Ezrin/Radixin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 67kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Moesin/Ezrin/Radixin. AA range:524-573 |
| Specificity | Moesin/Ezrin/Radixin Polyclonal Antibody detects endogenous levels of Moesin/Ezrin/Radixin protein. |
| Gene Name | MSN |
| Protein Name | Moesin |
| Other Name | MSN; / Moesin; / Membrane-organizing extension spike protein; / RDX; / Radixin; / EZR; / VIL2; / Ezrin; / Cytovillin; / Villin-2; / p81 |
| Species | Human |
| Gene ID | 4478;5962 |
| Uniprot | P26038;P35241;P15311, |
| Species.1 | Mouse |
| Gene ID.1 | 17698;19684;22350, |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID.2 | 81521;54319 |
| Uniprot.2 | O35763;P31977 |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasm, cytoskeleton . Apical cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, microvillus . Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By similarity). Localizes at the uropods of T lymphoblasts. . |
| Tissue Expression | In all tissues and cultured cells studied. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Human Diseases >> Cancer: overview >> MicroRNAs in cancer |
| Research Areas | >>Tight junction; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Measles; / >>Proteoglycans in cancer |
| Function | Function:Probably involved in connections of major cytoskeletal structures to the plasma membrane.,PTM:Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.,similarity:Contains 1 FERM domain.,subcellular location:Phosphorylated form is enriched in microvilli-like structures at apical membrane.,subunit:In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation (By similarity). Binds SLC9A3R1.,tissue specificity:In all tissues and cultured cells studied., |
