U-Blot® MYL9 Rabbit mAb
WR4259 Western blot analysis of lysates from Mouse liver cells, using Ferritin Heavy Chain Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Myosin Light Chain 2 |
| Reactivity | Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 20kD |
| MW(Observed) | 20kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0379R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MYL9 |
| Protein Name | Myosin regulatory light polypeptide 9 |
| Other Name | MYL9; / MLC2; / MRLC1; / MYRL2; / Myosin regulatory light polypeptide 9; / 20 kDa myosin light chain; / LC20; / MLC-2C; / Myosin RLC; / Myosin regulatory light chain 2; / smooth muscle isoform; / Myosin regulatory light chain 9; / Myosin regulatory light chain MRL |
| Species | Human |
| Gene ID | 2495 |
| Uniprot | P24844, |
| Species.1 | Mouse |
| Gene ID.1 | 98932 |
| Uniprot.1 | Q9CQ19, |
| Species.2 | Rat |
| Gene ID.2 | 25319 |
| Uniprot.2 | P19132 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Expressed in the liver. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Ferroptosis;Cellular Processes >> Cell growth and death >> Necroptosis |
| Research Areas | >>Ferroptosis; / >>Necroptosis; / >>Mineral absorption |
| Function | Function:Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.,miscellaneous:This chain binds calcium.,PTM:Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,tissue specificity:Smooth muscle tissues and in some, but not all, nonmuscle cells., |
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