Price range: $268.00 through $328.00
Background:
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | MyoD1 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 35kD |
| MW(Observed) | 45kD |
| Host Species | Mouse |
| Isotype | IgG2b, Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-500; WB 1:1000-5000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM-MYOD1 |
| Immunogen | Synthesized peptide derived from human MyoD1 AA range: 100-200 |
| Specificity | This antibody detects endogenous levels of MyoD1 protein. |
| Gene Name | MYOD1 BHLHC1 MYF3 MYOD |
| Protein Name | Myoblast determination protein 1 (Class C basic helix-loop-helix protein 1) (bHLHc1) (Myogenic factor 3) (Myf-3) |
| Other Name | MYOD1; / BHLHC1; / MYF3; / MYOD; / Myoblast determination protein 1; / Class C basic helix-loop-helix protein 1; / bHLHc1; / Myogenic factor 3; / Myf-3 |
| Species | Human |
| Gene ID | 4654 |
| Uniprot | P15172, |
| Species.1 | Mouse |
| Gene ID.1 | 17927 |
| Uniprot.1 | P10085, |
| Species.2 | Rat |
| Gene ID.2 | 337868 |
| Uniprot.2 | Q02346 |
| Cellular Localization | Nuclear |
| Tissue Expression | Muscle ,Skeletal muscle , |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia |
| Research Areas | >>Spinocerebellar ataxia |
| Function | Function:Involved in muscle differentiation (myogenic factor) . Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. ,online information:MyoD entry ,PTM:Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely , its deacetylation by SIRT1 inhibits its function. ,PTM:Ubiquitinated on the N-terminus; which is required for proteasomal degradation. ,similarity:Contains 1 basic helix-loop-helix (bHLH) domain. ,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1. , |
