Name: U-Blot® MYPT1 Rabbit mAb
SKU: WR4382
Price: 268 USD
Availability: InStock

U-Blot® MYPT1 Rabbit mAb

HEK293 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4382 HEK293 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

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50μl

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SKU: WR4382-50

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Product details

Background:Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosph

Specifications

Size	50μL/100μL
Target	MYPT1
Reactivity	Human
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	115kD
MW(Observed)	140kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0206R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PPP1R12A
Protein Name	Protein phosphatase 1 regulatory subunit 12A
Other Name	PPP1R12A; / MBS; / MYPT1; / Protein phosphatase 1 regulatory subunit 12A; / Myosin phosphatase-targeting subunit 1; / Myosin phosphatase target subunit 1; / Protein phosphatase myosin-binding subunit
Species	Human
Gene ID	57521
Uniprot	O14974,
Species.1	Mouse
Gene ID.1	17931
Uniprot.1	Q9DBR7
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm
Tissue Expression	Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>Autophagy - other; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Thermogenesis; / >>Insulin signaling pathway; / >>Shigellosis; / >>MicroRNAs in cancer
Function	Function:Regulates myosin phosphatase activity.,PTM:Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 6 ANK repeats.,subcellular location:Along actomyosin filaments and stress fibers.,subunit:PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.,

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U-Blot® MYPT1 Rabbit mAb

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U-Blot® MYPT1 Rabbit mAb

Product details

Specifications

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