U-Blot® NRAS Rabbit mAb
WR4130 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4130 Western blot analysis of lysates from 3T3-L1 cells, using NRAS Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
Specifications
| Size | 50μL/100μL |
| Target | N-RAS |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 22kD |
| MW(Observed) | 22kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0241R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | NRAS HRAS1 |
| Protein Name | GTPase NRas (Transforming protein N-Ras) |
| Other Name | |
| Species | Human |
| Gene ID | 4893 |
| Uniprot | P01111, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P08556, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q04970 |
| Cellular Localization | Membrane |
| Tissue Expression | |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Bladder cancer;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>Chemokine signaling pathway; / >>FoxO signaling pathway; / >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>Mitophagy - animal; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>Apoptosis; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Cellular senescence; / >>Axon guidance; / >>VEGF signaling pathway; / >>Apelin signaling pathway; / >>Gap junction; / >>Signaling pathways regulating pluripotency of stem cells; / >>C-type lectin receptor signaling pathway; / >>Natural killer cell mediated cytotoxicity; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Thermogenesis; / >>Long-term potentiation; / >>Neurotrophin signaling pathway; / >>Cholinergic synapse; / >>Serotonergic synapse; / >>Long-term depression; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>GnRH signaling pathway; / >>Estrogen signaling pathway; / >>Melanogenesis; / >>Prolactin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Oxytocin signaling pathway; / >>Relaxin signaling pathway; / >>GnRH secretion; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Growth hormone synthesis, secretion and action; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Alcoholism; / >>Hepatitis C; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Endometrial cancer; / >>Glioma; / >>Prostate cancer; / >>Thyroid cancer; / >>Melanoma; / >>Bladder cancer; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Non-small cell lung cancer; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Central carbon metabolism in cancer; / >>Choline metabolism in cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Lipid and atherosclerosis |
| Function |
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