Price range: $268.00 through $328.00
Background:The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],
| Size | 50μL/100μL |
| Target | NUMB |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 71kD |
| MW(Observed) | 71kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1264R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | NUMB |
| Protein Name | Protein numb homolog |
| Other Name | NUMB; / Protein numb homolog; / h-Numb; / Protein S171 |
| Species | Human |
| Gene ID | 8650 |
| Uniprot | P49757, |
| Species.1 | Mouse |
| Gene ID.1 | 18222 |
| Uniprot.1 | Q9QZS3, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q2LC84 |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side . Endosome membrane; Peripheral membrane protein; Cytoplasmic side . Localizes to perinuclear endosomes in an AAK1-dependent manner. . |
| Tissue Expression | B cells,Blood,Brain,Cervix carcinoma,Epithelium,Fetal brain,HeLa cells,Live |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Notch signaling pathway |
| Research Areas | >>Notch signaling pathway |
| Function | Function:Implicated in the control of cell fate decisions during development.,PTM:Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.,sequence Caution:Intron retention.,similarity:Contains 1 PID domain.,subunit:Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis., |
