Name: U-Blot® Oct-4 Rabbit mAb
SKU: WR4246
Price: 268 USD
Availability: InStock

U-Blot® Oct-4 Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4246 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

Western blot analysis of lysates from NCCIT cells, using 45569 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4246 Western blot analysis of lysates from NCCIT cells, using 45569 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4246-50

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Product details

Background:This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation withThe Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One ofThe AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013],

Specifications

Size	50μL/100μL
Target	Oct 3/4
Reactivity	Human, Mouse
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	39kD
MW(Observed)	45kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:1000-1:5000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0430R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	POU5F1
Protein Name	POU domain class 5 transcription factor 1
Other Name	POU5F1; / OCT3; / OCT4; / OTF3; / POU domain; / class 5, transcription factor 1; / Octamer-binding protein 3; / Oct-3; / Octamer-binding protein 4; / Oct-4; / Octamer-binding transcription factor 3; / OTF-3
Species	Human
Gene ID	5460
Uniprot	Q01860,
Species.1	Mouse
Gene ID.1	18999
Uniprot.1	P20263
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus
Tissue Expression	Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells
Research Areas	>>Signaling pathways regulating pluripotency of stem cells
Function	Function:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.,miscellaneous:Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.,online information:Oct-4 entry,PTM:Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.,similarity:Belongs to the POU transcription factor family. Class-5 subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 POU-specific domain.,subcellular location:Expressed in a diffuse and slightly punctuate pattern.,subunit:Interacts with UBE2I.,tissue specificity:Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.,