U-Blot® p90RSK (phospho Thr359/Ser363) Rabbit mAb
WR4393 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4393 MCF7 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:ribosomal protein S6 kinase A1(RPS6KA1) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Rsk-1 Phospho Thr359/Ser363 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 83kD |
| MW(Observed) | 83kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0755R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | RPS6KA1 MAPKAPK1A RSK1 |
| Protein Name | Ribosomal protein S6 kinase alpha-1 |
| Other Name | RPS6KA1; / MAPKAPK1A; / RSK1; / Ribosomal protein S6 kinase alpha-1; / S6K-alpha-1; / 90 kDa ribosomal protein S6 kinase 1; / p90-RSK 1; / p90RSK1; / p90S6K; / MAP kinase-activated protein kinase 1a; / MAPK-activated protein kinase 1a; / MAPKAP kinase 1a; / MAPKAP |
| Species | Human |
| Gene ID | 27086 |
| Uniprot | Q15418, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P18653, |
| Species.2 | Rat |
| Gene ID.2 | 297480 |
| Uniprot.2 | Q498D1 |
| Cellular Localization | Cytoplasm, Nucleus |
| Tissue Expression | Isoform 8 is specifically expressed in embryonic stem cells. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> MicroRNAs in cancer |
| Research Areas | >>MicroRNAs in cancer |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,cofactor:Magnesium.,enzyme regulation:Activated by multiple phosphorylations on threonine and serine residues.,Function:Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.,PTM:Autophosphorylated on Ser-380, as part of the activation process.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation., |
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