U-Blot® Paxillin Rabbit mAb
WR4156 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4156 Western blot analysis of lysates from Hela cells, using Paxillin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011],
Specifications
| Size | 50μL/100μL |
| Target | Paxillin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 65kD |
| MW(Observed) | 68kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0303R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PXN |
| Protein Name | Paxillin |
| Other Name | PXN; / Paxillin |
| Species | Human |
| Gene ID | 5829 |
| Uniprot | P49023, |
| Species.1 | Mouse |
| Gene ID.1 | 19303 |
| Uniprot.1 | Q8VI36, |
| Species.2 | Rat |
| Gene ID.2 | 360820 |
| Uniprot.2 | Q66H76 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Brain,Epithelium,Lung,Placenta,T-cell,Uterus, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Immune system >> Chemokine signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway |
| Research Areas | >>Chemokine signaling pathway; / >>VEGF signaling pathway; / >>Focal adhesion; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Bacterial invasion of epithelial cells; / >>Shigellosis; / >>Yersinia infection; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Human immunodeficiency virus 1 infection; / >>Viral carcinogenesis; / >>Proteoglycans in cancer |
| Function | Function:Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).,PTM:Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.,similarity:Belongs to the paxillin family.,similarity:Contains 3 LIM zinc-binding domains.,similarity:Contains 4 LIM zinc-binding domains.,subunit:Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5., |
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