Name: U-Blot® PCNA Rabbit mAb
SKU: WR4120
Price: 268 USD
Availability: InStock

U-Blot® PCNA Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4120 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4120 Mouse thymus

Immunohistochemical analysis of paraffin-embedded Human spleen tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4120 Immunohistochemical analysis of paraffin-embedded Human spleen tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Western blot analysis of lysates from HepG2 cells, using PCNA Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4120 Western blot analysis of lysates from HepG2 cells, using PCNA Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4120-50

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Product details

Background:The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	PCNA
Reactivity	Human, Mouse, Rat, Dog, Duck
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	29kD
MW(Observed)	36kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:1000-1:4000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0224R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PCNA
Protein Name	Proliferating cell nuclear antigen
Other Name	ATLD2; / cb16; / Cyclin; / DNA polymerase delta auxiliary protein; / etID36690.10; / fa28e03; / fb36g03; / HGCN8729; / MGC8367; / Mutagen-sensitive 209 protein; / OTTHUMP00000030189; / OTTHUMP00000030190; / PCNA; / Pcna/cyclin; / PCNA_HUMAN; / PCNAR; / Polymerase delta accessory protein; / Proliferating cell nuclear antigen; / wu:fa28e03; / wu:fb36g03
Species	Human
Gene ID	5111
Uniprot	P12004,
Species.1	Mouse
Gene ID.1	18538
Uniprot.1	P17918,
Species.2	Rat
Gene ID.2	25737
Uniprot.2	P04961
Cellular Localization	Nucleus
Tissue Expression	Tonsil/ Colon
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cellular community - eukaryotes >> Tight junction
Research Areas
Function	Disease:Antibodies are present in sera from patients with systemic lupus erythematosus.,Function:This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.,online information:PCNA entry,PTM:Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases RNF8 and SHPRH, which are required for DNA repair.,similarity:Belongs to the PCNA family.,subunit:Homotrimer. Interacts with KCTD10. Interacts with PPP1R15A (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH. Forms a ternary complex with DNTTIP2 and core histone. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B/WSTF; the interaction is direct.,

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U-Blot® PCNA Rabbit mAb

Product details

Specifications

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U-Blot® PCNA Rabbit mAb

Product details

Specifications

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