U-Blot® PD-L1 Mouse mAb
Ucallm_WM1785-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
Specifications
| Size | 50μL/100μL |
| Target | CD274 |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 33kD |
| MW(Observed) | 50kD |
| Host Species | Mouse |
| Isotype | Mouse IgG1/Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-1:100; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM015 |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1 |
| Protein Name | Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274) |
| Other Name | Programmed cell death 1 ligand 1; / PD-L1; / PDCD1 ligand 1; / Programmed death ligand 1; / B7 homolog 1; / B7-H1; / CD antigen CD274 |
| Species | Human |
| Gene ID | 29126 |
| Uniprot | Q9NZQ7, |
| Species.1 | Mouse |
| Gene ID.1 | 60533 |
| Uniprot.1 | Q9EP73 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein . Early endosome membrane; Single-pass type I membrane protein . Recycling endosome membrane; Single-pass type I membrane protein . Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation. .; [Isoform 1]: Cell membrane; Single-pass type I membrane protein .; [Isoform 2]: Endomembrane system; Single-pass type I membrane protein . |
| Tissue Expression | Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | >>Cell adhesion molecules; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer |
| Function | Function:Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.,induction:Up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. Up-regulated in B-cells activated by surface Ig cross-linking.,similarity:Belongs to the immunoglobulin superfamily. BTN/MOG family.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:Interacts with PDCD1.,tissue specificity:Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes., |
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