Name: U-Blot® PDGFR-α Rabbit mAb
SKU: WR4375
Price: 268 USD
Availability: InStock

Product details

Background:platelet derived growth factor receptor alpha(PDGFRA) Homo sapiens This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012],

Specifications

Size	50μL/100μL
Target	PDGFR-α
Reactivity	Human, Mouse, Rat
Applications	WB, IF, IP, ELISA
MW(Calculated)	123kD
MW(Observed)	190kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0591R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PDGFRA PDGFR2 RHEPDGFRA
Protein Name	Platelet-derived growth factor receptor alpha (PDGF-R-alpha) (PDGFR-alpha) (Alpha platelet-derived growth factor receptor) (Alpha-type platelet-derived growth factor receptor) (CD140 antigen-like family member A) (CD140a antigen) (Platelet-derived growth factor alpha receptor) (Platelet-derived growth factor receptor 2) (PDGFR-2) (CD antigen CD140a)
Other Name	Platelet-derived growth factor receptor alpha; / PDGF-R-alpha; / PDGFR-alpha; / Alpha platelet-derived growth factor receptor; / Alpha-type platelet-derived growth factor receptor; / CD140 antigen-like family member A; / CD140a antigen; / Platelet-derived growth factor alpha receptor; / Platelet-derived growth factor receptor 2; / PDGFR-2; / CD antigen CD140a
Species	Human
Gene ID	5156
Uniprot	P16234,
Species.1	Mouse
Gene ID.1	18595
Uniprot.1	P26618,
Species.2	Rat
Gene ID.2	25267
Uniprot.2	P20786
Cellular Localization	Cell membrane; Single-pass type I membrane protein . Cell projection, cilium . Golgi apparatus .
Tissue Expression	Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Prostate cancer;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>EGFR tyrosine kinase inhibitor resistance; / >>MAPK signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>Calcium signaling pathway; / >>Phospholipase D signaling pathway; / >>Endocytosis; / >>PI3K-Akt signaling pathway; / >>Focal adhesion; / >>Gap junction; / >>JAK-STAT signaling pathway; / >>Regulation of actin cytoskeleton; / >>Human cytomegalovirus infection; / >>Pathways in cancer; / >>MicroRNAs in cancer; / >>Glioma; / >>Prostate cancer; / >>Melanoma; / >>Central carbon metabolism in cancer; / >>Choline metabolism in cancer
Function	Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.