U-Blot® PDPK1 Rabbit mAb
WR4428 Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,
Specifications
| Size | 50μL/100μL |
| Target | PDPK1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 63kD |
| MW(Observed) | 58-68kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0647R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PDPK1 |
| Protein Name | 3-phosphoinositide-dependent protein kinase 1 |
| Other Name | PDPK1; / PDK1; / 3-phosphoinositide-dependent protein kinase 1; / hPDK1 |
| Species | Human |
| Gene ID | 5170 |
| Uniprot | O15530, |
| Species.1 | Mouse |
| Gene ID.1 | 18607 |
| Uniprot.1 | Q9Z2A0, |
| Species.2 | Rat |
| Gene ID.2 | 81745 |
| Uniprot.2 | O55173 |
| Cellular Localization | Membrane, Cytoplasm |
| Tissue Expression | Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> PPAR signaling pathway;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>Platinum drug resistance; / >>PPAR signaling pathway; / >>FoxO signaling pathway; / >>Sphingolipid signaling pathway; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Apoptosis; / >>Axon guidance; / >>Focal adhesion; / >>T cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Neurotrophin signaling pathway; / >>Insulin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Insulin resistance; / >>Aldosterone-regulated sodium reabsorption; / >>Toxoplasmosis; / >>Proteoglycans in cancer; / >>Chemical carcinogenesis - reactive oxygen species; / >>Endometrial cancer; / >>Prostate cancer; / >>Non-small cell lung cancer; / >>Choline metabolism in cancer; / >>Lipid and atherosclerosis |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously., |
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