Name: U-Blot® PGC1 β Rabbit mAb
SKU: WR4101
Price: 268 USD
Availability: InStock

U-Blot® PGC1 β Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4101 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

Western blot analysis of lysates from MCF7 cells, using PGC1 β Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4101 Western blot analysis of lysates from MCF7 cells, using PGC1 β Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4101-50

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Product details

Background:The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],

Specifications

Size	50μL/100μL
Target	PGC1 β
Reactivity	Human, Mouse, Rat
Applications	WB, IP, ELISA
MW(Calculated)	113kD
MW(Observed)	113kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000-1:5000; ELISA 1:5000-1:20000; IP 1:50-1:200,
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0194R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PPARGC1B
Protein Name	Peroxisome proliferator-activated receptor gamma coactivator 1-beta
Other Name	PPARGC1B PERC PGC1 PGC1B PPARGC1
Species	Human
Gene ID	133522
Uniprot	Q86YN6,
Species.1	Mouse
Gene ID.1	170826
Uniprot.1	Q8VHJ7,
Species.2	Rat
Gene ID.2	291567
Uniprot.2	Q811R2
Cellular Localization	Nucleus
Tissue Expression	Ubiquitous with higher expression in heart, brain and skeletal muscle.
Signaling_pathway
Research Areas
Function	Domain:Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors.,Function:Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure.,induction:Repressed by saturated fatty acids such as palmitate and stearate in skeletal muscle cells. Induced by insulin and reduced by aging in skeletal muscle biopsies. Down-regulated in type 2 diabetes mellitus subjects as well as in pre-diabetics.,polymorphism:Variation of PPARGC1B may contribute to the pathogenesis of obesity, with a widespread Ala-203 allele being a risk factor for the development of this common disorders.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with hepatocyte nuclear factor 4-alpha/HNF4A, Sterol regulatory binding transcription factor 1/SREBF1, PPAR-alpha/PPARA, thyroid hormone receptor beta/THRB and host cell factor/HCFC1. Interacts with estrogen-related receptor gamma/ESRRG and alpha/ESRRA (By similarity). Interacts with estrogen receptor alpha/ESR1.,tissue specificity:Ubiquitous with higher expression in heart, brain and skeletal muscle.,