U-Blot® PGC1 β Rabbit mAb
WR4101 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4101 Western blot analysis of lysates from MCF7 cells, using PGC1 β Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],
Specifications
| Size | 50μL/100μL |
| Target | PGC1 β |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IP, ELISA |
| MW(Calculated) | 113kD |
| MW(Observed) | 113kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:5000; ELISA 1:5000-1:20000; IP 1:50-1:200, |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0194R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PPARGC1B |
| Protein Name | Peroxisome proliferator-activated receptor gamma coactivator 1-beta |
| Other Name | PPARGC1B PERC PGC1 PGC1B PPARGC1 |
| Species | Human |
| Gene ID | 133522 |
| Uniprot | Q86YN6, |
| Species.1 | Mouse |
| Gene ID.1 | 170826 |
| Uniprot.1 | Q8VHJ7, |
| Species.2 | Rat |
| Gene ID.2 | 291567 |
| Uniprot.2 | Q811R2 |
| Cellular Localization | Nucleus |
| Tissue Expression | Ubiquitous with higher expression in heart, brain and skeletal muscle. |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors.,Function:Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure.,induction:Repressed by saturated fatty acids such as palmitate and stearate in skeletal muscle cells. Induced by insulin and reduced by aging in skeletal muscle biopsies. Down-regulated in type 2 diabetes mellitus subjects as well as in pre-diabetics.,polymorphism:Variation of PPARGC1B may contribute to the pathogenesis of obesity, with a widespread Ala-203 allele being a risk factor for the development of this common disorders.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with hepatocyte nuclear factor 4-alpha/HNF4A, Sterol regulatory binding transcription factor 1/SREBF1, PPAR-alpha/PPARA, thyroid hormone receptor beta/THRB and host cell factor/HCFC1. Interacts with estrogen-related receptor gamma/ESRRG and alpha/ESRRA (By similarity). Interacts with estrogen receptor alpha/ESR1.,tissue specificity:Ubiquitous with higher expression in heart, brain and skeletal muscle., |
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