PGP 9.5 [ABT-PGP9.5] mouse mAb
Ucallm_WM1444-50
Price range: $268.00 through $328.00
Product details
Background:
The molecular weight of PGP 9.5 protein is 27 kDa. It is highly expressed in normal neurons, widely distributed in neurons, nerve fibers and neuroendocrine cells of central and peripheral nervous system, and also expressed in renal tubules, Leydig cells, testicular spermatogonia, eggs and some cells in pregnant and non pregnant corpus luteum. It is often used in combination with Syn, CgA, CD56 and NSE for the study of neuroendocrine cells and their derived tumors.
Specifications
| Size | 50μL/100μL |
| Target | PGP9.5 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 25kD |
| MW(Observed) | 25kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM-PGP9.5 |
| Immunogen | Synthesized peptide derived from human PGP 9.5 AA range: 100-223 |
| Specificity | The antibody can specifically recognize human PGP 9.5 protein. In western blotting of Jurkat cell lysate, the antibody can label a 25 kDa band corresponding to PGP 9.5. |
| Gene Name | UCHL1 |
| Protein Name | Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) (Neuron cytoplasmic protein 9.5) (PGP 9.5) (PGP9.5) (Ubiquitin thioesterase L1) |
| Other Name | Epididymis luminal protein 117; / Epididymis secretory protein Li 53; / HEL 117; / HEL S 53; / NDGOA; / Neuron cytoplasmic protein 9.5; / OTTHUMP00000218137; / OTTHUMP00000218139; / OTTHUMP00000218140; / OTTHUMP00000218141; / Park 5; / PARK5; / PGP 9.5; / PGP9.5; / PGP95; / Protein gene product 9.5; / Ubiquitin C terminal esterase L1; / Ubiquitin C terminal hydrolase; / Ubiquitin C terminal hydrolase L1; / Ubiquitin carboxyl terminal esterase L1; / Ubiquitin carboxyl terminal hydrolase isozyme L1; / Ubiquitin carboxyl-terminal hydrolase isozyme L1; / Ubiquitin thioesterase L1; / Ubiquitin thiolesterase; / Ubiquitin thiolesterase L1; / UCH-L1; / UCHL1; / UCHL1_HUMAN |
| Species | Human |
| Gene ID | 7345 |
| Uniprot | P09936 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases |
| Research Areas | >>Parkinson disease; / >>Pathways of neurodegeneration - multiple diseases |
| Function | Catalytic activity:Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).,Disease:Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles.,Function:Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.,miscellaneous:In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.,online information:Ubiquitin carboxy-terminal hydrolase L1 entry,PTM:O-glycosylated.,similarity:Belongs to the peptidase C12 family.,subunit:Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.,tissue specificity:Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary., |
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