Name: Phospho Smad3 [S425] mouse Monoclonal Antibody [1D9]
SKU: WM1773
Price: 288 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

Specifications

Size	50μL/100μL
Target	Smad3 Phospho Ser425
Reactivity	Human, Mouse, Rat
Applications	IHC, IF
MW(Calculated)
MW(Observed)	52kD
Host Species	Mouse
Isotype
Conjugate/Modification	phosphate
Recommended Dilution Ratio	IHC 1:50-300; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Monoclonal
Clone Number	1D9
Immunogen	Synthetic Peptide of Phospho-Smad3(S425)
Specificity	The antibody detects endogenous Phospho-Smad3(S425) protein
Gene Name	SMAD3 MADH3
Protein Name	Mothers against decapentaplegic homolog 3 (MAD homolog 3) (Mad3) (Mothers against DPP homolog 3) (hMAD-3) (JV15-2) (SMAD family member 3) (SMAD 3) (Smad3) (hSMAD3)
Other Name	SMAD3; / MADH3; / Mothers against decapentaplegic homolog 3; / MAD homolog 3; / Mad3; / Mothers against DPP homolog 3; / hMAD-3; / JV15-2; / SMAD family member 3; / SMAD 3; / Smad3; / hSMAD3
Species	Human
Gene ID	4088
Uniprot	P84022,
Species.1	Mouse
Gene ID.1	17127
Uniprot.1	Q8BUN5,
Species.2	Rat
Gene ID.2	25631
Uniprot.2	P84025
Cellular Localization	Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .
Tissue Expression	Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Organismal Systems >> Immune system >> Th17 cell differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Immune disease >> Inflammatory bowel disease;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway
Research Areas	>>FoxO signaling pathway; / >>Cell cycle; / >>Endocytosis; / >>Cellular senescence; / >>Wnt signaling pathway; / >>TGF-beta signaling pathway; / >>Apelin signaling pathway; / >>Hippo signaling pathway; / >>Adherens junction; / >>Signaling pathways regulating pluripotency of stem cells; / >>Th17 cell differentiation; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Hepatitis B; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Colorectal cancer; / >>Pancreatic cancer; / >>Chronic myeloid leukemia; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Inflammatory bowel disease; / >>Diabetic cardiomyopathy
Function	Disease:Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].,Domain:The MH2 domain is sufficient to carry protein nuclear export.,Function:Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with Smad4.,subunit:Interacts with HGS. Interacts with NEDD4L in response to TGF-beta. Interacts with TTRAP (By similarity). Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4. Interacts with JUN/FOS, vitamin D receptor, homeobox protein TGIF and TGIF2, PEBP2-alpha C subunit, CREB-binding protein (CBP), p300, SKI, SNON, ATF2, SMURF2, AIP1, DACH1 and TGFB1I1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Found in a complex with SMAD2 and TRIM33 upon addition of TGF-beta. Interacts with SMAD2 and TRIM33. Found in a complex with SMAD3, Ran and XPO4. Interacts with XPO4. Interacts with LBXCOR1 and CORL2.,

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Phospho Smad3 [S425] mouse Monoclonal Antibody [1D9]

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Phospho Smad3 [S425] mouse Monoclonal Antibody [1D9]

Product details

Specifications

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