Name: U-Blot® PKC δ Rabbit mAb
SKU: WR4386
Price: 268 USD
Availability: InStock

Product details

Background:Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	PKC δ
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	78kD
MW(Observed)	78kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:500-1:2000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0605R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PRKCD
Protein Name	Protein kinase C delta type
Other Name	PRKCD; / Protein kinase C delta type; / Tyrosine-protein kinase PRKCD; / nPKC-delta
Species	Human
Gene ID	5580
Uniprot	Q05655,
Species.1	Mouse
Gene ID.1	18753
Uniprot.1	P28867,
Species.2	Rat
Gene ID.2	170538
Uniprot.2	P09215
Cellular Localization	Cytoplasm, Nucleus
Tissue Expression	Epithelium,Hippocampus,Liver,Platelet,Skin,
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Human Diseases >> Neurodegenerative disease >> Prion disease
Research Areas	>>Chemokine signaling pathway; / >>Autophagy - animal; / >>Vascular smooth muscle contraction; / >>NOD-like receptor signaling pathway; / >>C-type lectin receptor signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Neurotrophin signaling pathway; / >>Inflammatory mediator regulation of TRP channels; / >>GnRH signaling pathway; / >>Estrogen signaling pathway; / >>Type II diabetes mellitus; / >>Insulin resistance; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Prion disease; / >>Shigellosis; / >>Chemical carcinogenesis - reactive oxygen species; / >>Diabetic cardiomyopathy
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.,Domain:The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.,enzyme regulation:Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation.,Function:This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.,PTM:Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with PDK1, RAD9A, CDCP1 and MUC1.,

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U-Blot® PKC δ Rabbit mAb

Product details

Specifications

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U-Blot® PKC δ Rabbit mAb

Product details

Specifications

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