Price range: $268.00 through $328.00
Background:The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],
| Size | 50μL/100μL |
| Target | PLK1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 68kD |
| MW(Observed) | 68kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0679R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PLK1 PLK |
| Protein Name | PLK1 |
| Other Name | Serine/threonine-protein kinase PLK1; / Polo-like kinase 1; / PLK-1; / Serine/threonine-protein kinase 13; / STPK13 |
| Species | Human |
| Gene ID | 5347 |
| Uniprot | P53350, |
| Species.1 | Mouse |
| Gene ID.1 | 18817 |
| Uniprot.1 | Q07832, |
| Species.2 | Rat |
| Gene ID.2 | 25515 |
| Uniprot.2 | Q62673 |
| Cellular Localization | Nucleus |
| Tissue Expression | Placenta and colon. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>Cell cycle; / >>Oocyte meiosis; / >>Progesterone-mediated oocyte maturation |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,developmental stage:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.,enzyme regulation:Activated by serine and threonine phosphorylation.,Function:Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.,induction:By growth-stimulating agents.,PTM:Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.,PTM:Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 POLO box domains.,subunit:Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A.,tissue specificity:Placenta and colon., |
