Price range: $268.00 through $328.00
Background:
PMEL is a type I transmembrane glycoprotein which is specific in malanocyte. PMEL is abundantly expressed in melanosomes, and plays a central role in the biogenesis of melanosomes by involved in the maturation of melanosomes from stage I to II. It is highly expressed in all stages of melanoma progression, so the PMEL antibody can specifically labels melanoma cells.
| Size | 50μL/100μL |
| Target | HMB45 |
| Reactivity | Human, Mouse |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 70kD |
| MW(Observed) | 80kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM255 |
| Immunogen | Synthesized peptide derived from human PMEL AA range: 400-500 |
| Specificity | The antibody can specifically recognize human PMEL protein. |
| Gene Name | PMEL D12S53E PMEL17 SILV |
| Protein Name | 95 kDa melanocyte specific secreted glycoprotein; 95 kDa melanocyte-specific secreted glycoprotein; D12S53E; gp100; M-beta; ME20; ME20 M/ME20 S; ME20-M; ME20-S; ME20M; ME20M/ME20S; ME20S; Melanocyte lineage specific antigen GP100; Melanocyte protein mel 17; Melanocyte protein Pmel 17; Melanocyte protein Pmel 17 precursor; Melanocytes lineage-specific antigen GP100; Melanoma associated ME20 antigen; Melanoma gp100; Melanoma-associated ME20 antigen; Melanosomal matrix protein 17; Melanosomal matrix protein17; P1; p100; p26; PMEL 17; PMEL; PMEL_HUMAN; PMEL17; Premelanosome protein; Secreted melanoma-associated ME20 antigen; SI; SIL; SILV; Silver (mouse homolog) like; Silver homolog; Silver locus protein homolog; Silver, mouse, homolog of |
| Other Name | 95 kDa melanocyte specific secreted glycoprotein; / 95 kDa melanocyte-specific secreted glycoprotein; / D12S53E; / gp100; / M-beta; / ME20; / ME20 M/ME20 S; / ME20-M; / ME20-S; / ME20M; / ME20M/ME20S; / ME20S; / Melanocyte lineage specific antigen GP100; / Melanocyte protein mel 17; / Melanocyte protein Pmel 17; / Melanocyte protein Pmel 17 precursor; / Melanocytes lineage-specific antigen GP100; / Melanoma associated ME20 antigen; / Melanoma gp100; / Melanoma-associated ME20 antigen; / Melanosomal matrix protein 17; / Melanosomal matrix protein17; / P1; / p100; / p26; / PMEL 17; / PMEL; / PMEL_HUMAN; / PMEL17; / Premelanosome protein; / Secreted melanoma-associated ME20 antigen; / SI; / SIL; / SILV; / Silver; / Mouse homolog; / like; / Silver homolog; / Silver locus protein homolog; / Silver, Mouse, homolog of |
| Species | Human |
| Gene ID | |
| Uniprot | P40967, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q60696 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Skin/ Melanoma |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.,similarity:Belongs to the Pmel-17/NMB family.,similarity:Contains 1 PKD domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subcellular location:Probably product of proteolytic cleavage.,tissue specificity:Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas., |
