Name: U-Blot® PPAR-γ Rabbit mAb
SKU: WR4795
Price: 268 USD
Availability: InStock

Product details

Background:peroxisome proliferator activated receptor gamma(PPARG) Homo sapiens This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	PPAR-γ
Reactivity	Human, Mouse
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	58kD
MW(Observed)	53kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:500-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0357R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PPARG
Protein Name	Peroxisome proliferator-activated receptor gamma
Other Name	PPARG; / NR1C3; / Peroxisome proliferator-activated receptor gamma; / PPAR-gamma; / Nuclear receptor subfamily 1 group C member 3
Species	Human
Gene ID	5468
Uniprot	P37231,
Species.1	Mouse
Gene ID.1	19016
Uniprot.1	P37238
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus. Cytoplasm. Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. NOCT enhances its nuclear translocation.
Tissue Expression	Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Signaling_pathway	Organismal Systems >> Endocrine system >> PPAR signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Organismal Systems >> Aging >> Longevity regulating pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Neurodegenerative disease >> Huntington disease;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>PPAR signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Osteoclast differentiation; / >>Thermogenesis; / >>Non-alcoholic fatty liver disease; / >>Huntington disease; / >>Pathways in cancer; / >>Transcriptional misregulation in cancer; / >>Thyroid cancer; / >>Lipid and atherosclerosis
Function	Alternative products:Additional isoforms seem to exist,Disease:Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.,Disease:Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension.,Disease:Defects in PPARG may be associated with colon cancer.,Disease:Defects in PPARG may be associated with susceptibility to obesity [MIM:601665].,Disease:Variation in PPARG is associated with carotid intimal medial thickness 1 (CIMT1) [MIM:609338]. CIMT is a measure of atherosclerosis that is independently associated with traditional atherosclerotic cardiovascular disease risk factors and coronary atherosclerotic burden. 35 to 45% of the variability in multivariable-adjusted CIMT is explained by genetic factors.,Function:Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.,online information:Peroxisome proliferator-activated receptor entry,online information:The Singapore human mutation and polymorphism database,polymorphism:Genetic variation in PPARG may influence body mass index (BMI) [MIM:606641]. BMI reflects the amount of fat, lean mass, and body build.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Forms a heterodimer with the retinoic acid receptor RXRA called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes. Interacts with FAM120B (By similarity). Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 LXXLL motifs. Interacts with TGFB1I1. Interacts with DNTTIP2.,tissue specificity:Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.,