Name: PPP1A mouse mAb
SKU: WM0572
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	PP1α
Reactivity	Human
Applications	WB, IHC, ICC
MW(Calculated)
MW(Observed)	38kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000; ICC 1:300
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	9G9
Immunogen	Purified recombinant human PPP1A protein fragments expressed in E.coli.
Specificity	This antibody detects endogenous levels of PPP1A and does not cross-react with related proteins.
Gene Name	ppp1ca
Protein Name
Other Name	Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit; / Catalytic subunit; / MGC15877; / MGC1674; / PP 1A; / PP-1A; / PP1A; / PP1A_HUMAN; / PP1alpha; / PP2C ALPHA; / PP2CA; / Ppp1ca; / Protein Phosphatase 2C Alpha Isoform; / Serine threonine protein phosphatase PP1 alpha catalytic subunit; / Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1; / Serine/threonine-protein phosphatase PP1-alpha catalytic subunit.
Species	Human
Gene ID	5499
Uniprot	P62136,
Species.1	Mouse
Gene ID.1
Uniprot.1	P62137
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm . Nucleus . Nucleus, nucleoplasm . Nucleus, nucleolus . Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles. Shuttles toward the cytosol during infection with VEEV (PubMed:29769351). .
Tissue Expression	Colon carcinoma,Liver,Lung,Muscle,Pancreas,Placenta,Platele
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway
Research Areas	>>mRNA surveillance pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Oocyte meiosis; / >>Cellular senescence; / >>Adrenergic signaling in cardiomyocytes; / >>Vascular smooth muscle contraction; / >>Hippo signaling pathway; / >>Focal adhesion; / >>Platelet activation; / >>Long-term potentiation; / >>Dopaminergic synapse; / >>Inflammatory mediator regulation of TRP channels; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>Oxytocin signaling pathway; / >>Insulin resistance; / >>Amphetamine addiction; / >>Alcoholism; / >>Herpes simplex virus 1 infection; / >>Proteoglycans in cancer; / >>Diabetic cardiomyopathy
Function	Catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,enzyme regulation:The phosphatase activity of the PPP1R15A-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, a drug that protects cells from endoplasmic reticulum stress.,Function:Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.,online information:The things we forget -Issue 32 of March 2003,similarity:Belongs to the PPP phosphatase family.,similarity:Belongs to the PPP phosphatase family. PP-1 subfamily.,subunit:PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. Interacts with PPP1R9A and PPP1R9B. Part of a complex containing PPP1R15B, PP1 and NCK1/2 (By similarity). Interacts with PPP1R7. PPP1R15A and PPP1R15B mediate binding to EIF2S1. Interacts with HHV-1 ICP34.5.,