Price range: $268.00 through $328.00
Background:prohibitin(PHB) Homo sapiens This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
| Size | 50μL/100μL |
| Target | Prohibitin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 30kD |
| MW(Observed) | 30kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0204R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PHB |
| Protein Name | Prohibitin |
| Other Name | PHB; / Prohibitin |
| Species | Human |
| Gene ID | 5245 |
| Uniprot | P35232, |
| Species.1 | Mouse |
| Gene ID.1 | 18673 |
| Uniprot.1 | P67778, |
| Species.2 | Rat |
| Gene ID.2 | 25344 |
| Uniprot.2 | P67779 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Widely expressed in different tissues. |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Levels of expression in fibroblasts decrease heterogeneously during cellular aging.,Disease:Mutated in sporadic breast cancer.,Function:Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.,induction:Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. Also induced following inhibition of mitochondrial protein synthesis by thiamphenicol.,similarity:Belongs to the prohibitin family.,subunit:Interacts with PHB2.,tissue specificity:Widely expressed in different tissues., |
