Price range: $268.00 through $328.00
Background:
This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | RanBP9 |
| Reactivity | Human |
| Applications | WB, ICC, IP |
| MW(Calculated) | |
| MW(Observed) | 78kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000; ICC 1:300 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 20B8 |
| Immunogen | Purified recombinant human RanBP9 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of RanBP9 and does not cross-react with related proteins. |
| Gene Name | ranbp9 |
| Protein Name | |
| Other Name | B cell antigen receptor Ig beta associated protein 1; / BPM 90; / BPM L; / BPM-L; / BPM90; / BPML; / IBAP 1; / Imp 9; / Importin 9; / Novel centrosomal protein RanBPM; / RAN binding protein 9; / Ran binding protein centrosomal; / Ran Binding Protein in the Microtubule organizing center; / Ran binding protein M; / Ran BP9; / Ran-binding protein 9; / Ran-binding protein M; / RANB9_HUMAN; / RanBP 7; / RANBP 9; / RanBP7; / RanBP9; / RanBPM. |
| Species | Human |
| Gene ID | 10048 |
| Uniprot | Q96S59, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P69566 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Nucleus . Cell membrane; Peripheral membrane protein . The unphosphorylated form is predominantly cytoplasmic. A phosphorylated form is associated with the plasma membrane. . |
| Tissue Expression | Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells. |
| Signaling_pathway | |
| Research Areas | |
| Function | Caution:According to some authors (PubMed:9817760) RANBP9 would be located in centrosomes and involved in microtubule assembly, but other authors infirmed these results in (PubMed:11470507).,Domain:The SPRY domain mediates the interaction with MET, AR, and CDC2L1.,Function:May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).,PTM:Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the RANBP9/10 family.,similarity:Contains 1 B30.2/SPRY domain.,similarity:Contains 1 CTLH domain.,similarity:Contains 1 LisH domain.,subcellular location:A phosphorylated form is associated with the plasma membrane.,subunit:Interacts with NGFR and DDX4 (By similarity). Interacts with GTP-bound Ran, AR, CDC2L1/p110C, CALB1, S100A7, USP11, MKLN1, SOS1 or SOS2, C20orf11, and FMR1. Interacts with the Dyrk kinases HIPK2, DYRK1A, and DYRK1B. Interacts with TP73 isoform Alpha but not with TP53. Interacts with the HGF receptor MET and the integrins ITGB1 and ITGB2, but not with ITGAL. Part of a complex consisting of RANBP9, MKLN1 and C20ORF11. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5.,tissue specificity:Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells., |
