Name: Recombinant CD8 Fc-Fused Nanobody (VF0550)
SKU: WN0530
Price: 365 USD
Availability: InStock

Product details

Background:The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found forThis gene. [provided by RefSeq, Nov 2011]

Specifications

Size	100μL
Target	CD8
Reactivity	Human
Applications	ELISA, FC
MW(Calculated)
MW(Observed)
Host Species
Isotype	IgG2a,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	ELISA 1:5000-100000
Formulation	Phosphate-buffered solution
Purification	Recombinant Expression and Affinity purified
Storage	-15°C to -25°C/1 year(Avoid freeze / thaw cycles)
Concentration	Please check the information on the tube
Clonality
Clone Number	VF0627
Immunogen	Purified recombinant Human CD8
Specificity	This recombinant monoclonal antibody can detects endogenous levels of CD8 protein.
Gene Name	CD8A MAL
Protein Name	T-cell surface glycoprotein CD8 alpha chain (T-lymphocyte differentiation antigen T8/Leu-2) (CD antigen CD8a)
Other Name	T-cell surface glycoprotein CD8 alpha chain; / T-lymphocyte differentiation antigen T8/Leu-2; / CD antigen CD8a; / CD8 nanobody
Species	Human
Gene ID	925
Uniprot	P01732
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	[Isoform 1]: Cell membrane; Single-pass type I membrane protein. CD8A localizes to lipid rafts only when associated with its partner CD8B. .; [Isoform 2]: Secreted .
Tissue Expression	Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.
Signaling_pathway	Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Human Diseases >> Immune disease >> Primary immunodeficiency;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas
Function	Disease:Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.,Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.,online information:CD8 entry,online information:CD8A mutation db,PTM:All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.,