Recombinant IL-1B Fc-Fused Nanobody (VF0165)
Ucallm_WN0616
$365.00
Product details
Background:IL-1β in humans and mice does not encode a typical signal peptide and, as a result, newly synthesized pro-IL-1β accumulates within the cytoplasm of activated monocytes and macrophages . Conversion of the inactive pro-IL-1β to its mature form requires the proteolytic action of IL-1β-converting enzyme (ICE), also termed caspase-1 . Secretion of mature IL-1β from LPS-activated monocytes/macrophages is not a constitutive process. These cells must encounter a secondary stimulus that specifically activates the posttranslational processing events . Moreover, owing to its pro-inflammatory nature, IL-1β is regarded as a tumor-promoting cytokine. In fact, enhanced tumor metastasis and angiogenesis has been observed under the influence of IL-1β . IL-1β is able to facilitate tumor progression in murine models of lung cancer. In addition, upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor .
Specifications
| Size | 100μL |
| Target | IL-1β |
| Reactivity | Human |
| Applications | ELISA |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | |
| Isotype | IgG2a,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | ELISA 1:5000-100000; Flow Cyt 1-2μg/Test |
| Formulation | Phosphate-buffered solution |
| Purification | Recombinant Expression and Affinity purified |
| Storage | -15°C to -25°C/1 year(Avoid freeze / thaw cycles) |
| Concentration | Please check the information on the tube |
| Clonality | |
| Clone Number | VF0220 |
| Immunogen | Purified recombinant Human IL-1B |
| Specificity | This recombinant monoclonal antibody can detects endogenous levels of IL-1B protein. |
| Gene Name | IL1B IL1F2 |
| Protein Name | Interleukin-1 beta (IL-1 beta) (Catabolin) |
| Other Name | IL-1; / IL1F2; / IL1beta; / IL1-BETA; / Catabolin antibody; / H1 antibody; / IFN beta inducing factor antibody; / IL 1 antibody; / IL 1 beta antibody; / IL-1 beta antibody; / IL1 antibody; / IL1 BETA antibody; / IL1B antibody; / IL1B_HUMAN antibody; / IL1F2 antibody; / Interleukin 1 beta antibody; / Interleukin 1 beta precursor antibody; / interleukin 1; / beta antibody; / Interleukin-1 beta antibody; / OAF antibody; / Osteoclast activating factor antibody; / OTTHUMP00000162031 antibody; / Preinterleukin 1 beta antibody; / Preinterleukin beta antibody; / Pro interleukin 1 beta antibody; / IL-1β; / IL-1B nanobody |
| Species | Human |
| Gene ID | 3553 |
| Uniprot | P01584 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144). In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744). Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744). In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059). . |
| Tissue Expression | Expressed in B-cell lines and T-lymphocytes. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Inflammatory bowel disease;Human Diseases >> Immune disease >> Graft-versus-host disease;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction |
| Research Areas | |
| Function | Interleukin (IL)-1β is a cytokine with a key role in the pathophysiology of local and systemic inflammation. IL-1β induces cytokine, chemokine, proinflammatory molecule secretion, and adhesion molecule expression in diverse cells. |
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