Recombinant mCherry Fc-Fused Nanobody (VF0083)
Ucallm_WN0641
$365.00
Product details
Background:mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Specifications
| Size | 100μL |
| Target | mCherry-Tag |
| Reactivity | Human |
| Applications | WB |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | |
| Isotype | IgG2a,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | ELISA 1:5000-100000 |
| Formulation | Phosphate-buffered solution |
| Purification | Recombinant Expression and Affinity purified |
| Storage | -15°C to -25°C/1 year(Avoid freeze / thaw cycles) |
| Concentration | Please check the information on the tube |
| Clonality | |
| Clone Number | VF0083 |
| Immunogen | Recombinant Protein of mCherry-Tag |
| Specificity | The antibody detects mCherry tag fusion proteins. |
| Gene Name | ERBB2 |
| Protein Name | Receptor tyrosine-protein kinase erbB-2 |
| Other Name | mCherry; / mCherry Tag |
| Species | Human |
| Gene ID | |
| Uniprot | |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Early endosome . Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794). .; [Isoform 2]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm. Nucleus. |
| Tissue Expression | Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. |
| Signaling_pathway | |
| Research Areas | >>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>Platinum drug resistance; / >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Calcium signaling pathway; / >>HIF-1 signaling pathway; / >>PI3K-Akt signaling pathway; / >>Focal adhesion; / >>Adherens junction; / >>Tight junction; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Pancreatic cancer; / >>Endometrial cancer; / >>Prostate cancer; / >>Bladder cancer; / >>Non-small cell lung cancer; / >>Breast cancer; / >>Gastric cancer; / >>Central carbon metabolism in cancer |
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Disease:Defects in ERBB2 are associated with familial glioma of brain [MIM:137800]; also called glioblastoma multiforme. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas.,Disease:Defects in ERBB2 are associated with gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC).,Disease:Defects in ERBB2 are associated with lung cancer [MIM:211980]; also called adenocarcinoma of lung.,Disease:Defects in ERBB2 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.,Function:Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.,online information:ERBB2 entry,polymorphism:There are fours alleles due to the variations in positions 654 and 655. Allele B1 (Ile-654/Ile-655) has a frequency of 0.782; allele B2 (Ile-654/Val-655) has a frequency of 0.206; allele B3 (Val-654/Val-655) has a frequency of 0.012.,PTM:Ligand-binding increases phosphorylation on tyrosine residues.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248. Interacts with MUC1. Stimulation by heregulin (HRG) in breast cancer cell lines induces binding of MUC1 with gamma-catenin., |
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