Name: Rho A Mouse mAb [4E12]
SKU: WM1107
Price: 268 USD
Availability: InStock

Product details

Background:
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],

Specifications

Size	50μL/100μL
Target	Rho A
Reactivity	Human, Mouse, Rat
Applications	IHC, WB
MW(Calculated)
MW(Observed)	22kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC1:50-200; WB 1:1000-2000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Monoclonal
Clone Number	4E12
Immunogen	Synthesized peptide derived from human Rho A
Specificity	This antibody detects endogenous levels of Rho A at Human, Mouse,Rat
Gene Name	RHOA ARH12 ARHA RHO12
Protein Name	Rho A
Other Name	Transforming protein RhoA; / Rho cDNA clone 12; / h12
Species	Human
Gene ID	387
Uniprot	P61586,
Species.1	Mouse
Gene ID.1	11848
Uniprot.1	Q9QUI0,
Species.2	Rat
Gene ID.2	117273
Uniprot.2	P61589
Cellular Localization	Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex . Midbody. Cell projection, lamellipodium . Cell projection, dendrite . Nucleus . Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner. .
Tissue Expression	Adipose tissue,Brain,Colon,Mammary cancer,Oesophageal carcinoma,Placenta,Re
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>Ras signaling pathway; / >>Rap1 signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>Endocytosis; / >>mTOR signaling pathway; / >>Vascular smooth muscle contraction; / >>Wnt signaling pathway; / >>TGF-beta signaling pathway; / >>Axon guidance; / >>Focal adhesion; / >>Adherens junction; / >>Tight junction; / >>Platelet activation; / >>NOD-like receptor signaling pathway; / >>C-type lectin receptor signaling pathway; / >>T cell receptor signaling pathway; / >>Leukocyte transendothelial migration; / >>Neurotrophin signaling pathway; / >>Regulation of actin cytoskeleton; / >>Oxytocin signaling pathway; / >>Parathyroid hormone synthesis, secretion and action; / >>Pancreatic secretion; / >>Bacterial invasion of epithelial cells; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Yersinia infection; / >>Tuberculosis; / >>Human cytomegalovirus infection; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Colorectal cancer; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	cofactor:Magnesium.,Domain:The basic-rich region is essential for yopT recognition and cleavage.,Function:Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.,PTM:Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.,PTM:Substrate for botulinum ADP-ribosyltransferase.,similarity:Belongs to the small GTPase superfamily. Rho family.,subunit:Interacts with RGNEF (By similarity). Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation.,

Please login to post questions

Rho A Mouse mAb [4E12]

Product details

Specifications

About Us

Support

Order

Menu

Rho A Mouse mAb [4E12]

Product details

Specifications

Related products

U-Blot™ Phosphate Buffered Saline with Tween 20 (PBS-T), pH 7.4

U-Blot® GAPDH Rabbit mAb

U-Blot® VEGF Receptor 1 Rabbit mAb

U-Blot® MMP7 Rabbit mAb

U-Blot® CDC37 (Phospho Ser13) Rabbit mAb

U-Blot® CDK6 Rabbit mAb

Customer Reviews

Menu